Panel

Hypothesis-driven quantitative fluorescence microscopy – the importance of reverse-thinking in experimental design

One of the challenges in trendy fluorescence microscopy is to reconcile the typical utilization of microscopes as exploratory devices with their rising and quickly increasing function as a quantitative instruments. The contribution of microscopy to observational biology will stay monumental owing to the enhancements in acquisition velocity, imaging depth, decision and biocompatibility of trendy imaging devices. However, the use of […]

Non-classical crystallisation pathway directly observed for a pharmaceutical crystal via liquid phase electron microscopy

Non-classical crystallisation (NCC) pathways are broadly accepted, nonetheless there’s conflicting proof relating to the intermediate phases of crystallisation, how they manifest and additional turn into crystals. Evidence from direct observations is particularly missing for small natural molecules, as distinguishing these low-electron dense entities from their comparable liquid-phase environment presents signal-to-noise ratio and distinction challenges. Here, Liquid Phase Electron Microscopy (LPEM) […]

Nonlinear Optical Microscopy: From Fundamentals to Applications in Live Bioimaging