Expected use
The EMAb ELISA is designed for use in the detection of anti-endometrial antibodies in human serum. Endometriosis is a common gynecological disease, often leading to infertility, the infertility rate is as high as 30-40%. Studies have shown that the incidence of endometriosis is related to an autoimmune disease, the patient is often accompanied by a series of changes in immune function. High titers of EMAb can often be detected in patient serum, peritoneal fluid, and endometrial tissue and is therefore considered a sensitive indicator in clinical diagnosis.
Summary and explanation
Purified endometrial antigen is coated onto the surface of the microwells. Patient serum is added to the wells and EMAb, if present, binds to the antigen during incubation. After washing the wells to remove unbound sample, the HRP-conjugated human antibody is added. Unbound conjugate is removed by a subsequent wash step. A TMB reagent solution is then added to the microwells. The catalytic reaction of the enzyme conjugate stops at a specific time. The intensity of the color generated is proportional to the amount of EMAb in the sample. Product details
