Expected use
The AoAb ELISA is designed for use in the detection of anti-ovarian antibodies in human serum. Anti-ovarian antibody usually appears in the serum of premature aging women, in patients with infertility and abortion, its positive rate (42.4-52.2%) is significantly higher than the positive rate of normal pregnant women (3, 2%), indicating that the existence of AoAb is closely related to infertility and abortion.
Summary and explanation
The purified ovarian antigen is coated onto the surface of the microwells. Patient serum is added to the wells and AoAb, if present, binds to the antigen during incubation. After washing the wells to remove unbound sample, the HRP-conjugated human antibody is added. Unbound conjugate is removed by a subsequent wash step. A TMB reagent solution is then added to the microwells. The catalytic reaction of the enzyme conjugate stops at a specific time. The intensity of the color generated is proportional to the amount of AoAb in the sample.
