Intended use
The Abuse Integrated Cup is a rapid visual immunoassay for the qualitative, presumptive detection of any combination of of abuse in human urine specimens.
Summary and Explanation
The of Abuse Integrated Cup is an immunoassay based on the principle of competitive binding. that may be present in the urine specimen compete against their respective conjugate for binding sites on their specific antibody. During testing, a portion of the urine specimen migrates upward by capillary action. A , if present in the urine specimen below its cut-off concentration, will not saturate the binding sites of its specific antibody. The antibody will then react with the -protein conjugate and a visible colored line will appear in the test line region of the corresponding strip. The presence of above the cut-off concentration in the urine specimen will saturate all the binding sites of the antibody. Therefore, no colored line will form in the test line region.
A -positive urine specimen will not generate a colored line in the specific test line region of the strip because of competition, while a -negative urine specimen will generate a line in the test line region because of the absence of competition. To serve as a procedural control, a colored line will always appear at the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred. Adulteration is the tampering of a urine specimen with the intention of altering the test results. The use of adulterants can cause false negative results in tests by either interfering with the screening test and / or destroying the present in the urine.
Dilution may also be employed in an attempt to produce false negative test results. One of the best ways to test for adulteration or dilution is to determine certain urinary characteristics such as Creatinine, pH, and Specific Gravity and to detect the presence of Glutaraldehyde, Nitrite and Oxidants / Pyridinium Chlorochromate in urine.
