Expected use
The ANA Antibody ELISA is designed for use in the detection of ANA antibodies to the virus in human serum. Systemic autoimmune diseases are characterized by the presence of antinuclear autoantibodies (ANA). Higher ANA titers are highly indicative of lupus erythematosus, although they also appear in some other autoimmune diseases such as drug-induced lupus erythematosus, overlap syndrome, mixed conjunctive. tissue disorder, scleroderma, polymyositis and dermatomyositis, Sjögren's syndrome, rheumatoid arthritis, autoimmune hepatitis, Hashimoto's, and myasthenia gravis.
Summary and explanation
The purified ANA antigen is coated onto the surface of the microwells. Patient serum is added to the wells and the ANA antibody, if present, binds to the antigen during incubation. After washing the wells to remove unbound sample, the HRP-conjugated human antibody is added. Unbound conjugate is removed by a subsequent wash step. A TMB reagent solution is then added to the microwells. The catalytic reaction of the enzyme conjugate stops at a specific time. The intensity of the color generated is proportional to the amount of ANA antibody in the sample.
