The high capacity cDNA reverse transcription kit contains all the components necessary for the quantitative conversion of up to 2 µg of total RNA to single stranded cDNA in a single 20 µL reaction. The high throughput cDNA reverse transcription kit offers very high quality single stranded cDNA from 0.02 to 2 µg of total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Subsequent applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA files.
Features of this kit include:
• Linear amplification of target for real-time PCR
• Higher performance and precision than other cDNA synthesis kits at a fraction of the cost
• 10 times greater dynamic range than other kits
Extensively tested with a variety of templates
Quantitative first strand synthesis of all RNA species is achieved with the use of random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNA expressed at low levels. CRNA can also be efficiently generated from in vitro transcription of cDNA.