Expected use

The HSV-2 IgG or IgM ELISA is designed for use in the detection of IgG or IgM against the herpes simplex virus HSV-2 in human serum or plasma.

Summary and explanation

The purified ds-DNA antigen is coated onto the surface of the microwells. Patient serum or plasma is added to the wells and the ds-DNA antibody, if present, binds to the antigen during incubation. After washing the wells to remove unbound sample, the HRP-conjugated human antibody is added.

Unbound conjugate is removed by a subsequent wash step. A TMB reagent solution is then added to the microwells. The catalytic reaction of the enzyme conjugate stops at a special time. The intensity of the color generated is proportional to the amount of ds-DNA antibody in the sample.