Fast Probe Master Mix for Real-Time PCR (5000 Reactions)

Product Information

Product name: Fast Probe Master Mix (5000 rxn)

Catalog number: 0037-#31005-2

Pack size: 50 × 1 mL

Reaction capacity: 5000 reactions, as listed; actual capacity depends on reaction volume.

Application: Probe-based real-time PCR (qPCR)

Enzyme: Cheetah™ chemically modified hot-start Taq DNA polymerase

Probe compatibility: Oligonucleotide probes, including TaqMan® and MGB probes

Origin: Animal

Shelf life: 12 months, as listed

Dry ice: No

Hazardous: No

Log in to view pricing and ordering options.

Login for Price

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

What Is Fast Probe Master Mix?

Fast Probe Master Mix is a reagent formulation designed for probe-based quantitative polymerase chain reaction (qPCR). Real-time PCR monitors DNA amplification during successive cycles using fluorescence generated by a sequence-specific probe. This approach allows researchers to detect and quantify target nucleic acids using appropriate standards, controls, and validated assay conditions.

According to the supplied product description, the master mix contains buffer, deoxynucleotide triphosphates (dNTPs), magnesium chloride (MgCl₂), and Cheetah™ Taq, a chemically modified hot-start DNA polymerase. Combining these components simplifies reaction preparation and supports consistent qPCR workflows.

How Probe-Based Real-Time PCR Works

Probe-based qPCR uses target-specific primers together with a fluorescent oligonucleotide probe. During amplification, a probe-dependent detection mechanism produces a fluorescence signal that can be measured by a real-time PCR instrument.

The resulting amplification curve and quantification cycle (Cq) can help estimate target abundance. Accurate interpretation depends on assay efficiency, template quality, appropriate controls, and the use of a suitable calibration or analysis method.

Key Product Features

• Probe-based detection: Designed for real-time PCR assays using fluorescent oligonucleotide probes.
• Hot-start chemistry: Contains chemically modified Cheetah™ Taq DNA polymerase.
• Integrated formulation: Includes buffer, dNTPs, MgCl₂, and hot-start enzyme.
• Rapid activation: The supplied description reports full enzyme activation in approximately two minutes under recommended conditions.
• High-throughput format: Listed for 5000 reactions, depending on the volume used per reaction.
• Probe compatibility: Designed for compatible TaqMan® and MGB probe assays.

Applications of Probe-Based qPCR

• DNA target detection and quantification.
• Gene expression analysis when combined with an appropriate reverse-transcription workflow.
• Genotyping and allele discrimination with compatible probe assays.
• Analysis of selected genetic targets in molecular biology research.
• Microbial or pathogen target detection in appropriately validated research assays.
• High-throughput nucleic acid analysis and comparative studies.

Experimental performance depends on primer and probe design, template quality, reaction conditions, instrument settings, and appropriate controls. Assays should be validated before results are interpreted.

TaqMan® and MGB Probes Explained

TaqMan® probes commonly contain a fluorescent reporter and a quencher. During target amplification, probe cleavage by the polymerase's 5′ nuclease activity separates the reporter from the quencher, allowing fluorescence to increase.

Minor groove binder (MGB) probes contain a minor groove binder group that can improve binding stability. This can support the design of shorter probes with useful sequence specificity. Compatibility and performance depend on the probe chemistry and assay design.

Technical Specifications

Experimental Considerations

• Follow the manufacturer's instructions for reaction setup, enzyme activation, and thermal cycling.
• Confirm whether the selected formulation contains ROX reference dye. The supplied description indicates that versions with and without ROX are available.
• Include suitable no-template and positive controls to monitor contamination and amplification performance.
• Use validated primers and probes and optimize conditions for the target and instrument.
• Store the master mix under the manufacturer's recommended conditions and minimize unnecessary freeze-thaw cycles.

Frequently Asked Questions

What is Fast Probe Master Mix used for?

It is formulated for probe-based real-time PCR, allowing researchers to monitor DNA amplification through fluorescence.

Which probes can be used with this master mix?

The supplied product description identifies TaqMan® and MGB probes. Confirm compatibility with the specific probe chemistry and assay protocol.

What is the role of Cheetah™ Taq?

Cheetah™ Taq is a chemically modified hot-start DNA polymerase designed to limit premature activity during reaction setup and support rapid activation under specified conditions.

How many reactions does the pack support?

The product is listed for 5000 reactions. The actual number depends on the amount of master mix used per reaction.