ELISA KITS · IMMUNOLOGY · CANCER RESEARCH
Human S100A12 (S100 Calcium Binding Protein A12) ELISA Kit
A sandwich ELISA kit for the quantitative detection of human S100A12 in serum, plasma, and other biological fluids.
Product Overview
The Human S100A12 ELISA Kit is designed for the quantitative measurement of S100 calcium-binding protein A12 (S100A12) in human biological samples. This immunoassay uses a sandwich ELISA format and a colorimetric detection method to measure S100A12 concentrations within the specified analytical range.
S100A12 is a member of the S100 family of calcium-binding proteins and is associated with inflammatory responses and immune signaling. This kit is suitable for research investigating inflammation-related pathways, immunology, cancer biology, and cellular signal transduction.
Key Product Features
- Designed for human S100A12 detection.
- Sandwich enzyme-linked immunosorbent assay (ELISA) format.
- Colorimetric detection method.
- Suitable for serum, plasma, and other biological fluids.
- 96-well plate format for laboratory research.
- Assay completion time of approximately 3 hours and 30 minutes.
Technical Specifications
| Product Name | Human S100A12 ELISA Kit |
| Catalog Number | 0617-EKE62868 |
| Target | S100A12 (S100 Calcium Binding Protein A12) |
| Species Reactivity | Human (Homo sapiens) |
| Accession Number | P80511 |
| Kit Size | 96 Tests |
| Assay Type | Sandwich ELISA |
| Detection Method | Colorimetric |
| Detection Range | 7.81–500 pg/mL |
| Sensitivity | 6.5 pg/mL |
| Sample Types | Serum, plasma, and other biological fluids |
| Assay Time | Approximately 3 hours and 30 minutes |
| Field of Research | Cancer, immunology, and signal transduction |
| Shipping Conditions | Ice packs; dry ice not required |
| Storage Conditions | 2–8°C for 12 months; consult the product manual for long-term storage |
| Hazardous Status | Not classified as hazardous according to the supplied product information |
Principle of the Sandwich ELISA
The sandwich ELISA method relies on the specific recognition of the target protein by antibodies. S100A12 in the sample is captured by an immobilized antibody and detected by a second antibody. A color-producing reaction generates an optical signal that can be measured using a suitable microplate reader.
The signal is compared with a standard curve to estimate the S100A12 concentration in the tested samples. Follow the manufacturer's instructions for reagent preparation, incubation conditions, washing steps, calibration, and result interpretation.
Research Applications
- Investigation of S100A12 expression in human biological samples.
- Studies of inflammatory and immune signaling pathways.
- Research into cancer-associated biological processes.
- Analysis of S100A12 levels in experimental and translational research.
- Exploration of calcium-binding proteins and their biological functions.
Sample Types and Handling
This kit is intended for use with serum, plasma, and other compatible biological fluids. Sample collection, processing, dilution, and storage should follow the manufacturer's validated protocol. Confirm sample compatibility and any required pretreatment in the product manual before beginning the assay.
Storage and Shipping
- Recommended storage: 2–8°C for 12 months, as specified in the supplied product information.
- Long-term storage: Refer to the manufacturer's manual.
- Shipping: Ice packs.
- Dry ice: Not required.
Ordering Information
Product: Human S100A12 ELISA Kit
Catalog Number: 0617-EKE62868
Pack Size: 96 Tests
Availability: Stock items typically ship within 24–48 hours; non-stock items typically require 2–6 weeks. Confirm availability when ordering.
Important Research Disclaimer
For Research Use Only (RUO). Not for use in diagnostic procedures. This product is intended for laboratory research and is not intended for clinical diagnosis or treatment decisions.