RNA STAT-60: Rapid RNA Isolation Reagent
Introduction
RNA isolation is a critical first step for gene expression studies, RT-qPCR, RNA-seq, and transcriptomic analysis. High-quality, intact RNA ensures reliable downstream results. RNA STAT-60 is a monophasic solution of phenol and guanidine thiocyanate specifically designed for rapid and efficient extraction of total RNA from cells and tissues.
Product Overview
Product Name: RNA STAT-60
SKU: CS-502
Size: 500 mL
RNA STAT-60 simplifies RNA purification by combining cell lysis, RNase inactivation, and RNA stabilization in a single solution. Its formulation allows researchers to extract high-purity RNA suitable for sensitive molecular biology applications.
Key Features & Advantages
✔️ Efficient RNA Extraction
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Rapid isolation from cells, tissues, and biological samples
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Preserves intact RNA, minimizing degradation
✔️ High Purity & Yield
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Phenol/guanidine-based chemistry removes proteins and DNA
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Compatible with RT-qPCR, Northern blotting, RNA-seq
✔️ Ready-to-Use Reagent
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Eliminates multiple washing steps
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Suitable for small and large-scale extractions
✔️ Versatile Applications
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Cell biology: mRNA analysis, gene expression
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Clinical research: RNA biomarker studies
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Molecular biology: Transcriptomics and functional genomics
Mechanism of Action
RNA STAT-60 employs the classic guanidinium thiocyanate–phenol method:
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Cell/Tissue Lysis: Denatures proteins and disrupts cellular membranes
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RNase Inactivation: Protects RNA from enzymatic degradation
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Phase Separation: Chloroform addition separates RNA into the aqueous phase
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RNA Precipitation: Isopropanol recovers high-purity RNA
This workflow ensures rapid, reproducible RNA isolation suitable for high-throughput studies.
Typical Workflow
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Homogenize sample in RNA STAT-60
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Add chloroform, mix, and centrifuge
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Collect the aqueous phase
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Precipitate RNA with isopropanol
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Wash with ethanol and dissolve in RNase-free water
RNA STAT-60 delivers intact RNA in less than 30 minutes, ideal for fast-paced labs.
Packaging Advantage
500 mL bottle is suitable for:
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Routine lab use
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High-volume RNA extractions
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Large-scale transcriptomic studies
Conclusion
RNA STAT-60 is a trusted reagent for efficient RNA isolation, providing high yield, purity, and reproducibility. Its ready-to-use formula saves time while maintaining RNA integrity, making it indispensable for molecular biology, genomics, and clinical research applications.