Expected use
The ds-DNA antibody ELISA is designed for use in the detection of ds-DNA antibodies to the virus in human serum. The detection of ds-DNA antibodies is very important for the diagnosis and treatment of lupus erythematosus, they also appear in some cases. other autoimmune diseases, but this type of disease is also SLE overlap syndrome in general. Therefore, the detection of the ds-DNA antibody is one of the standards for lupus erythematosus.
Summary and explanation
The purified ds-DNA antigen is coated onto the surface of the microwells. Patient serum is added to the wells and the ds-DNA antibody, if present, binds to the antigen during incubation. After washing the wells to remove unbound sample, the HRP-conjugated human antibody is added. Unbound conjugate is removed by a subsequent wash step.
A TMB reagent solution is then added to the microwells. The catalytic reaction of the enzyme conjugate stops at a special time. The intensity of the color generated is proportional to the amount of ds-DNA antibody in the sample.
