Mouse anti Monkey IgA antibody, clone IgA5-3B recognizes monkey IgA and does not cross-react with IgG or IgM.
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Goat Serum, Monkey IgA and Monkey Serum. No reaction was observed against Monkey IgG or Monkey IgM. Specificity was confirmed by ELISA at less than 1% cross reactivity against other monkey heavy or light chain isotypes.
Immunogen:
Monkey IgA alpha heavy chain
Physical properties:
Antibody format: IgG
Physical form:
Supplied in 0.1 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Species Reactivity:
Non-human primate
Host/Isotype:
Mouse / IgG1
Class:
Monoclonal
Type:
Secondary Antibody
Clone:
IgA5-3B
Conjugate:
Biotin
Form:
Liquid
Concentration:
0.5 mg/mL
Purification:
Protein A
Storage buffer:
PBS
Contains:
<0.1% sodium azide
Target:
IgA
Antibody Form:
Whole Antibody
Target Information:
Anti-Monkey secondary antibodies are affinity-purified antibodies with well-characterized specificity for non-human primate (e.g. monkey) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Storage Information:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Applications:
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting:
MCA2553 detects a band of approximately 55kDa under reducing conditions.
Disclaimer:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
