Expected use
The ASA ELISA is designed for use in the detection of anti-sperm antibodies in human serum. ASA positive rate is usually 10-30% in infertility patients, serum, especially obstructive azoospermia patients, ASA positive rate can be as high as 60%, high ASA titer is the reason vital that can result in autoimmune infertility. Detection of ASA offers a valuable prognostic indicator for the treatment of infertility.
Summary and explanation
The purified sperm antigen is coated onto the surface of the microwells. Patient serum is added to the wells and ASA, if present, binds to the antigen during incubation. After washing the wells to remove unbound sample, the HRP-conjugated human antibody is added. Unbound conjugate is removed by a subsequent wash step. A TMB reagent solution is then added to the microwells. The catalytic reaction of the enzyme conjugate stops at a specific time. The intensity of the color generated is proportional to the amount of ASA in the sample.
