Expected use
The TB IgG ELISA is designed for use in the detection of TB IgG against tuberculosis in human serum or plasma. Tuberculosis (TB) is considered a serious disease in general. It can infect tissues and organs, such as the lymph nodes, urinary system, reproductive system, skin, intestinal system, and even the central nervous system. TB is characterized by the presence of TB IgG in human serum or plasma.
Summary and explanation
The purified TB antigen is coated onto the surface of the microwells. Patient serum is added to the wells and TB IgG, if present, binds to the antigen during incubation. After washing the wells to remove unbound sample, the HRP conjugated human IgG antibody is added. Unbound conjugate is removed by a subsequent wash step.
A TMB reagent solution is then added to the microwells. The catalytic reaction of the enzyme conjugate stops at a specific time. The intensity of the color generated is proportional to the amount of TB IgG in the sample.